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Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya

Plant pesticides are a complementary means to other control methods and an alternative to chemical control. The identification of potential plants that can be used in plant protection against pests is increasing enormously, but the availability of bioformulations is low. The new products developed for plant protection aim to protect them against a larger range of pathogens and include elicitors. The objective of this study is to produce an optimal emulsion bioformulation based on neem (Azadirachta indica A. Juss.), Sida (Sida acuta Burm. F.) and calcium, that can be used for plant protection against pests. The surface plot of the four factor simplex lattice designs data help to construct significant linear models for stability and activity. The best formulation was selected and optimize. The four Factor mixture designs and stability as well as activity models showed that the best formulation has neem oil (No) 12% (v/v), neem aqueous extract (Ne) 9% (w/v), sida weed hydroalcoholic extract (Se) 9% (w/v), Oyster shell’s calcium-rich extract (Ca) 6% (w/v) and tween-80 (Tw) 24% (v/v) with the production process “AQ + (Tw+No)”. Therefore, the optimize formulation has No 12% (v/v), Ne 9% (w/v), Se 9% (w/v), Ca 4.5%(w/v), calcium oxide (CaO 1.5% (w/v) and Tw 24% (v/v) with the production process “(Tw+No) + AQ”. The best formulation and the optimize one at ambient temperature have 100% of stability and a significant dose dependent activity (P <0.05) against plant pathogens. Apply optimize formulation at 1% on the detached cocoa leaves before inoculation with Phytophthora megakarya reduces disease severity index from 4.2 to 1.5. These results suggest that our models and bioformulation can be useful for T. cocoa protection against P. megakarya, the causal agent of black pod disease.

Theobroma cocoa L., Phytophthora megakarya, Bioformulation of Fungicide, Neem, Sida Weed, Oyster Shell

Ebenezer Tatiekam Foka, Paul Martial Téné Tayo, Tatiana Flore Magni Pacha, Aristide Dzelamonyuy, Sylvain Leroy Kamdem Sado, et al. (2023). Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya. Journal of Plant Sciences, 11(6), 171-181.

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1. G. Lescuyer, L. Boutinot, P. Goglio et al., “Analyse de la chaîne de valeur du cacao au Cameroun,” Rapport pour l'Union Européenne, DG DEVCO. Value Chain Analysis for Development Project (VCA4D CTR 2016/375-804),” p. 127, 2020.
2. M. Ndoumbe-Nkeng, C. Cilas, E. Nyemb et al., “Impact of Removing Diseased Pods on Cacao Black Pod Caused by Phytophthora megakarya and on Cacao Production in Cameroon,” Journal of Crop Protection. vol. 23, pp. 415–424, 2004.
3. J. B. Mbarga, G. M. T. Hoopen, J. Kuate et al., “Trichoderma Asperellum: A potential biocontrol agent for Pythium myriotylum causal agent of cocoyam (Xanthosoma sagittifolium) root rot disease in Cameroon,” Crop Protection, vol. 36, pp. 18–22, 2012.
4. P. M. Tene-Tayo, A. Dzelamonyuy, D. Omokolo Ndoumou et al., “Enhancement of Theobroma cacao seedling growth and tolerance to Phytophthora megakarya by heat-treated oyster shell powder,” American Journal of Plant Sciences, vol. 10, pp. 578-594, 2019.
5. A. Dzelamonyuy, P. M. Tene-Tayo, E. R. Ngotcho et al., “Development of a powder formulation of Streptomyces cameroonensis and evaluation of its biocontrol and growth promoting activities against Phytophthora megakarya (causal agent of black pod disease) for two cocoa hybrids from Cameroon,” Journal of Microbiology and Biotechnology, vol. 32 no. 2, pp. 160-169, 2022.
6. Guomin Yang, Yanling Yao & Na Li. (2022). Knowledge, Attitudes and Practices (KAP) Related to the Coronavirus Disease 2019 (COVID-19) in a Village in Southern China: A Cross-sectional Survey. World Journal of Public Health (1). doi: 10.11648/J.WJPH.20220701.14 A. Jamiołkowska, “Natural Compounds as elicitors of plant resistance against diseases and new biocontrol strategies,” Agronomy, vol. 10, no. 2, p 173, 2020.
7. K. Biswas, I. Chattopadhyay, R. K. Banerjee et al., “Biological activities and medicinal properties of neem (Azadirachta indica),” Current science, vol. 82, no. 11, pp 1336-1345, 2002.
8. C. Wanren and L. Hua, “A brief review of the extraction technologies of azadirachtin, a plant-derived bio-pesticide,” African Journal of Engineering Research, vol. 6, no. 1, pp. 15-8, 2018.
9. S. Mohideen, E. Sasikala and V. Gopal, “Pharmacognostic studies on Sida acuta Burm. f.,” Ancient science of life, vol. 22, no. 1, pp. 57-66, 2002.
10. O. T. Tcheghebe, A. J. Seukep and F. N. Tatong, “Ethnomedicinal uses, phytochemical and pharmacological profiles, and toxicity of Sida acuta Burm. f.,” The Pharma Innovation Journal. vol. 6, no. 6, pp 01-06, 2017.
11. D. Karou, A. Savadogo, A. Canini et al. “Antibacterial activity of alkaloids from Sida acuta,” African Journal of biotechnology. vol. 4, no. 12, pp. 1452-7, 2005.
12. I. R. Iroha, E. S. Amadi, A. C. Nwuzo et al., “Evaluation of the antimicrobial avtivity of extracts of S. acuta against clinical isolates of Staphylococcus aueus isolated from human immunodeficiency virus/acquired immunodeficiency syndrome patients,” Research Journal of Pharmacology. Vol. 3, no. 2, pp. 22-25, 2009.
13. C. Nisha, P. Bhawana and M. H. Fulekar, “Antimicrobial Potential of Green Synthesized Silver nanoparticles using Sida acuta leaf extract,” Nano Science and Nano Technology. Vol. 11, no. 1, pp. 111-119, 2017.
14. B. Maia, S. Arcaro, M. Souza et al., “Characterization of sand casting and oyster shells as potential sources of raw material to produce soda-lime glasses,” Chemical Engineering and Transformation Journal, vol. 43, pp. 1795-1800, 2015.
15. N. Prabhakar and S. R. N. P. Jayashankar, “Relevance of Calcium Nutrition in Present Day Agriculture technology refinement for biochar production and evaluation of its effect on soil health and crop productivity view project waste management view project,” Advances in Life Sciences, vol. 5, pp. 2526–2530, 2016.
16. A. S. Djabou-Mouafi, Y. Qin, T. Boudjeko et al. “Effects of calcium and magnesium fertilization on antioxidant 2 activities during cassava postharvest physiological deterioration,” Crop Science, vol. 58, pp. 1–8, 2018.
17. S. E. Ranf, P. Lennart, J. Pascal et al., “Interplay between calcium signalling and early signalling elements during defence responses to microbe- or damage-associated molecular patterns,” Plant Journal, vol. 68, pp. 100–113., 2011.
18. F. Lesueur, “Élaboration de formulations à base d’extraits de neem (Azadirachta indica A. Juss) pour la protection de la pomme de terre (Solanum tuberosum L.) contre le Myzus persicae, un puceron colonisateur et vecteur de virus circulants et non circulants,” p 139. 2006.
19. J. R. Kuiate, K. M. L. Brigitte, R. K. Jules et al., “Screening of Some Plants Used in the Cameroonian Folk Medicine for the Treatment of Infectious Diseases,” International Journal of Biology, vol. 3, no. 4, pp. 13-21.
20. M. Gaonkar and A. C. Innovative, “Application of eggshell as fertilizer and calcium supplement tablet,” International Journal of Innovative Research in Science, Engineering and Technology, vol. 5, no. 3, pp. 3520–5, 2016.
21. J. Nelson, T. E. Y. S. Johnson, P. S. Vichangal et al., “Formulation of sweet flag oil (Acorus calamus) nanoemulsion by spontaneous emulsification method for the management of Sitophilus oryzae,” International Journal of Chemical Studies, vol. 7, no. 3, pp. 2072–6, 2019.
22. S. Limsuwan and S. P. Voravuthikunchai, “Bactericidal, Bacteriolytic, and Antibacterial Virulence Activities of Boesenbergia pandurata (Roxb) Schltr Extract against Streptococcus pyogenes,” Tropical Journal of Pharmaceutical Research, vol. 12, no. 6, pp. 1023–8, 2013.
23. Y. Zhang, P. Smith, S. N. Maximova et al., “Application of glycerol as a foliar spray activates the defense response and enhances disease resistance of Theobroma cacao,” Molecular Plant Pathology, vol. 16, no. 1, pp. 27–37, 2015.
24. S. Nyassé, C. Cilas, C. Herail et al., “Leaf inoculation as an early screening test for cocoa (Theobroma cacao L.) resistance to Phytophthora black pod disease,” Crop Protection, vol. 14, no. 8, pp; 657–63, 1995.
25. D. Paulin, M. Ducamp and P. Lachenaud, “New sources of resistance to Phytophthora megakarya identified in wild cocoa tree populations of French Guiana,” Crop Protection, vol. 27, no. 7, pp. 1143–7. 2008.
26. D. Srijita, “Biopesticides: an ecofriendly approach for pest control, “World Journal of Pharmacy and Pharmaceutical Sciences, vol. 4, no. 6, pp. 250–65, 2015.
27. G. M. Ten Hoopen and U. Krauss, “Biological Control of Cocoa Disease,” Springer, Cham, pp 511-566. 2016.
28. R. S. Ghotbi, M. Khatibzadeh and S. Kordbacheh, “Preparation of Neem Seed Oil Nanoemulsion,” In: Proceedings of the 5 th International Conference on Nanotechnology: Fundamentals and Applications, Pp 11-13, 2014.
29. M. Bhattacharya and S. Dixit, “Emulsification of Neem oil: effect of polar solvents and chain length of surfactant,” International Journal of chemical and pharmaceutical sciences. vol. 3, no. 12, pp. 2175–9, 2015.
30. K. R. S. Namasivayam, F. Lazar, H. Sanjana et al. “Effect of polymer-based surface coating on droplet size and potential pharmacological properties of neem oil,” IOAB Journal, vol. 8, pp. 1–6, 2017.
31. P. Nwankpa, O. Chukwuemeka, G. Uloneme et al. “Phyto-nutrient composition and antioxidative potential of ethanolic leaf extract of Sida acuta in wistar albino rats,” African Journal of Biotechnology, vol. 14, no. 49, pp. 3264–9, 2015.
32. J. Pohe and T. Agneroh, “L’huile des graines de neem, un fongicide alternatif à l’oxyde de cuivre dans la lutte contre la pourriture brune des cabosses de cacaoyer en Côte d’Ivoire,” Journal of Applied Biosciences, vol. 62, pp. 4644–4652, 2013.
33. A. Khaled and R. Mohamed, “Effet de composés calciques inorganiques sur le développement in vitro de moisissures isolées d’agrumes après la récolte,” Bulletin de la Société Royale des Sciences de Liège, vol. 85, pp. 263–75, 2016.
34. P. M. Téné-Tayo, C. A. Ewane, P. O. Effa et al., “Effects of chitosan and snail shell powder on cocoa (Theobroma cacao L.) growth and resistance against black pod disease caused by Phytophthora megakarya,” African journal of Plant Science, vol. 11, no. 8, pp. 331-340, 2017.