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Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar

Received: 19 September 2015     Accepted: 7 October 2015     Published: 19 October 2015
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Abstract

In order to continue the research of natural compounds of interest such as pesticides and therapeutic molecules in endemic species of Albizia from Madagascar, potentials of Albizia odorata seed extract were assessed. A toxic saponin (saponoside), named Albodorine, was isolated by extraction with hot ethanol or distilled water followed by purification procedure comprising n-butanol partition, precipitation by aceton-diethyl ether (50/50), Sephadex LH-20 gel chromatography and silica gel chromatography. All these methods were guided by toxicity tests on mice and homogeneity tests by Thin Layer Chromatography (TLC). Albodorine was thermostable, soluble in water and organic solvents and tasted bitter. Its acidic hydrolysis released glucose, arabinose and rhamnose. Tested on different experimental animal models, it was toxic to warm and cold blooded animals. In mouse, when intraperitoneally administered, it caused acute intoxication mainly presented as hyperpnea, ataxia and terminal seizures before the animal died. Its LD50 was about 9 mg/kg of mouse body weight by intraperitoneal route. In different organs, it caused histopathological lesions characterized by vascular congestions and important hemorrhage in liver, lungs and kidneys. In vitro, it reduced the heart rate and force of contraction of isolated rat atria. It had hemolytic activity. Albodorine showed toxicological properties that could be exploited under certain conditions for the control of harmful organisms.

Published in Journal of Plant Sciences (Volume 3, Issue 5)
DOI 10.11648/j.jps.20150305.14
Page(s) 264-271
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Albizia odorata, Toxin, Albodorine, Saponin, Hemolysis, Histopathology, Isolated Atria, Inotropic Effect

References
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Cite This Article
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    Clara Fredeline Rajemiarimoelisoa, Danielle Aurore Doll Rakoto, Hanitra Ranjana Randrianarivo, Victor Louis Jeannoda. (2015). Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar. Journal of Plant Sciences, 3(5), 264-271. https://doi.org/10.11648/j.jps.20150305.14

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    ACS Style

    Clara Fredeline Rajemiarimoelisoa; Danielle Aurore Doll Rakoto; Hanitra Ranjana Randrianarivo; Victor Louis Jeannoda. Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar. J. Plant Sci. 2015, 3(5), 264-271. doi: 10.11648/j.jps.20150305.14

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    AMA Style

    Clara Fredeline Rajemiarimoelisoa, Danielle Aurore Doll Rakoto, Hanitra Ranjana Randrianarivo, Victor Louis Jeannoda. Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar. J Plant Sci. 2015;3(5):264-271. doi: 10.11648/j.jps.20150305.14

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  • @article{10.11648/j.jps.20150305.14,
      author = {Clara Fredeline Rajemiarimoelisoa and Danielle Aurore Doll Rakoto and Hanitra Ranjana Randrianarivo and Victor Louis Jeannoda},
      title = {Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar},
      journal = {Journal of Plant Sciences},
      volume = {3},
      number = {5},
      pages = {264-271},
      doi = {10.11648/j.jps.20150305.14},
      url = {https://doi.org/10.11648/j.jps.20150305.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20150305.14},
      abstract = {In order to continue the research of natural compounds of interest such as pesticides and therapeutic molecules in endemic species of Albizia from Madagascar, potentials of Albizia odorata seed extract were assessed. A toxic saponin (saponoside), named Albodorine, was isolated by extraction with hot ethanol or distilled water followed by purification procedure comprising n-butanol partition, precipitation by aceton-diethyl ether (50/50), Sephadex LH-20 gel chromatography and silica gel chromatography. All these methods were guided by toxicity tests on mice and homogeneity tests by Thin Layer Chromatography (TLC). Albodorine was thermostable, soluble in water and organic solvents and tasted bitter. Its acidic hydrolysis released glucose, arabinose and rhamnose. Tested on different experimental animal models, it was toxic to warm and cold blooded animals. In mouse, when intraperitoneally administered, it caused acute intoxication mainly presented as hyperpnea, ataxia and terminal seizures before the animal died. Its LD50 was about 9 mg/kg of mouse body weight by intraperitoneal route. In different organs, it caused histopathological lesions characterized by vascular congestions and important hemorrhage in liver, lungs and kidneys. In vitro, it reduced the heart rate and force of contraction of isolated rat atria. It had hemolytic activity. Albodorine showed toxicological properties that could be exploited under certain conditions for the control of harmful organisms.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar
    AU  - Clara Fredeline Rajemiarimoelisoa
    AU  - Danielle Aurore Doll Rakoto
    AU  - Hanitra Ranjana Randrianarivo
    AU  - Victor Louis Jeannoda
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    DO  - 10.11648/j.jps.20150305.14
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 264
    EP  - 271
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20150305.14
    AB  - In order to continue the research of natural compounds of interest such as pesticides and therapeutic molecules in endemic species of Albizia from Madagascar, potentials of Albizia odorata seed extract were assessed. A toxic saponin (saponoside), named Albodorine, was isolated by extraction with hot ethanol or distilled water followed by purification procedure comprising n-butanol partition, precipitation by aceton-diethyl ether (50/50), Sephadex LH-20 gel chromatography and silica gel chromatography. All these methods were guided by toxicity tests on mice and homogeneity tests by Thin Layer Chromatography (TLC). Albodorine was thermostable, soluble in water and organic solvents and tasted bitter. Its acidic hydrolysis released glucose, arabinose and rhamnose. Tested on different experimental animal models, it was toxic to warm and cold blooded animals. In mouse, when intraperitoneally administered, it caused acute intoxication mainly presented as hyperpnea, ataxia and terminal seizures before the animal died. Its LD50 was about 9 mg/kg of mouse body weight by intraperitoneal route. In different organs, it caused histopathological lesions characterized by vascular congestions and important hemorrhage in liver, lungs and kidneys. In vitro, it reduced the heart rate and force of contraction of isolated rat atria. It had hemolytic activity. Albodorine showed toxicological properties that could be exploited under certain conditions for the control of harmful organisms.
    VL  - 3
    IS  - 5
    ER  - 

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Author Information
  • Department of Pharmacy, Faculty of Medicine, Antananarivo, University of Antananarivo, Madagascar

  • Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar

  • Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar

  • Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar

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