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An Efficient Method for High Quality RNA Extraction from Moringa oleifera

Received: 19 February 2017     Accepted: 28 February 2017     Published: 28 March 2017
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Abstract

Moringa oleifera, the Miracle Tree is rich in all nutrients and minerals. It has prominent distribution of secondary metabolites like polysaccharides, phenols and mucilage, which makes extraction of RNA quite difficult. A high quality and pure RNA is prerequisite for the study of high through put transcriptomics and functional genomics. A protocol for isolation of highly qualitative and quantitative RNA from M. oleifera was optimized by comparing five different methods like Trizol, Guanidine hydrochloride, combined Trizol and Guanidine hydrochloride, modified CTAB and hot phenol method. The combined Guanidine hydrochloride and Trizol method gave good yield and pure RNA based on the absorbance A260 value 2.0. The model plant Nicotiana tabacum served as a positive control in which the Trizol method yielded a good quality and quantity RNA. The present study is a preliminary step for studying the function and expression pattern of the genes. This is the first report on the comparison of different RNA extraction methods in M. oleifera to our knowledge.

Published in Journal of Plant Sciences (Volume 5, Issue 2)
DOI 10.11648/j.jps.20170502.14
Page(s) 68-74
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Moringa oleifera, Nicotiana tabacum, RNA Isolation, Trizol, Guanidine Hydrochloride, RT-PCR

References
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Cite This Article
  • APA Style

    Preethi Praba Umesh, Mohd Akram Ansari, Ganapathi Sridevi. (2017). An Efficient Method for High Quality RNA Extraction from Moringa oleifera. Journal of Plant Sciences, 5(2), 68-74. https://doi.org/10.11648/j.jps.20170502.14

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    ACS Style

    Preethi Praba Umesh; Mohd Akram Ansari; Ganapathi Sridevi. An Efficient Method for High Quality RNA Extraction from Moringa oleifera. J. Plant Sci. 2017, 5(2), 68-74. doi: 10.11648/j.jps.20170502.14

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    AMA Style

    Preethi Praba Umesh, Mohd Akram Ansari, Ganapathi Sridevi. An Efficient Method for High Quality RNA Extraction from Moringa oleifera. J Plant Sci. 2017;5(2):68-74. doi: 10.11648/j.jps.20170502.14

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  • @article{10.11648/j.jps.20170502.14,
      author = {Preethi Praba Umesh and Mohd Akram Ansari and Ganapathi Sridevi},
      title = {An Efficient Method for High Quality RNA Extraction from Moringa oleifera},
      journal = {Journal of Plant Sciences},
      volume = {5},
      number = {2},
      pages = {68-74},
      doi = {10.11648/j.jps.20170502.14},
      url = {https://doi.org/10.11648/j.jps.20170502.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20170502.14},
      abstract = {Moringa oleifera, the Miracle Tree is rich in all nutrients and minerals. It has prominent distribution of secondary metabolites like polysaccharides, phenols and mucilage, which makes extraction of RNA quite difficult. A high quality and pure RNA is prerequisite for the study of high through put transcriptomics and functional genomics. A protocol for isolation of highly qualitative and quantitative RNA from M. oleifera was optimized by comparing five different methods like Trizol, Guanidine hydrochloride, combined Trizol and Guanidine hydrochloride, modified CTAB and hot phenol method. The combined Guanidine hydrochloride and Trizol method gave good yield and pure RNA based on the absorbance A260 value 2.0. The model plant Nicotiana tabacum served as a positive control in which the Trizol method yielded a good quality and quantity RNA. The present study is a preliminary step for studying the function and expression pattern of the genes. This is the first report on the comparison of different RNA extraction methods in M. oleifera to our knowledge.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - An Efficient Method for High Quality RNA Extraction from Moringa oleifera
    AU  - Preethi Praba Umesh
    AU  - Mohd Akram Ansari
    AU  - Ganapathi Sridevi
    Y1  - 2017/03/28
    PY  - 2017
    N1  - https://doi.org/10.11648/j.jps.20170502.14
    DO  - 10.11648/j.jps.20170502.14
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 68
    EP  - 74
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20170502.14
    AB  - Moringa oleifera, the Miracle Tree is rich in all nutrients and minerals. It has prominent distribution of secondary metabolites like polysaccharides, phenols and mucilage, which makes extraction of RNA quite difficult. A high quality and pure RNA is prerequisite for the study of high through put transcriptomics and functional genomics. A protocol for isolation of highly qualitative and quantitative RNA from M. oleifera was optimized by comparing five different methods like Trizol, Guanidine hydrochloride, combined Trizol and Guanidine hydrochloride, modified CTAB and hot phenol method. The combined Guanidine hydrochloride and Trizol method gave good yield and pure RNA based on the absorbance A260 value 2.0. The model plant Nicotiana tabacum served as a positive control in which the Trizol method yielded a good quality and quantity RNA. The present study is a preliminary step for studying the function and expression pattern of the genes. This is the first report on the comparison of different RNA extraction methods in M. oleifera to our knowledge.
    VL  - 5
    IS  - 2
    ER  - 

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Author Information
  • Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India

  • Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India

  • Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India

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