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The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin

Received: 25 June 2018     Accepted: 11 July 2018     Published: 6 August 2018
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Abstract

Ex situ conservation of genetic resources remains a challenge for preservation of vegetatively propagated species such as sweet potato. The objective of this study was to establish the protocol based on the encapsulation-dehydration technique for short term preservation of endangered sweet potato landraces produced in Benin. Thus, the evaporative dehydration duration on silicagel was previously determinated on the empty beads which were made using alginate sodium (3%) and calcium chloride (1.32M). Then, the young shoots of two sweet potato landraces (Koïdokpon and Dokoui carotte) growing in the screen house were cutted aseptically and desinfected with 10% sodium hypochlorite. The apices were excised on stereoscope and were encapsulated prior their dehydration on silicagel during 5 and 6h. The encapsulated apices were finally conserved in eppendorf tubes at 2°C in batches for 15 days and 90 days. The encapsulated apices were cultured in MS medium supplemented with 0.15 mg/l BAP, 0.2 mg/l NAA, 0.08 mg/l GA3 and 80 mg/l adenine sulfate. The survival and the regeneration rates were then evaluated. At our experimentation condition, the dehydration duration which allowed around 20% water content of the beads was 6h. For the encapsulated apices, the highest survival rates (59.26% and 37.04%) and the highest regeneration rate (37.04% and 11.11%) were recorded respectivelly with the landraces ''Koïdokpon'' and ''Dokoui carotte'' when the apices were dehydrated by 6h and stored for 15 days. The regeneration rates decreased according to the stored duration. Significant difference was noted on the regeneration of apices for the landraces tested. This method can be used to preserve the endangered sweet potato landraces and other species during at least three months without subcultures. It also reduce the cost of conservation in terms of consumables and permit better genotype stability during the storage.

Published in Journal of Plant Sciences (Volume 6, Issue 3)
DOI 10.11648/j.jps.20180603.13
Page(s) 93-100
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Sweet Potato, Ex situ Preservation, Artificial Seeds, Shoot Apices, Benin

References
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[24] Clavero-Ramirez, I., Galvez-Farfan, J., Lopez-Aranda, J. and Gonzalez-Benito, M. (2005) Apex cryopreservation of several strawberry genotypes by two encapsulation-dehydration methods. CryoLetters 26(1): 17-24. Engelmann, F. (2011) Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cellular & Developmental Biology-Plant 47(1): 5-16.
[25] Dumet, D., Korie, S. and Adeyemi, A. (2009) Cryobanking cassava germplasm at IITA. In International Symposium on Cryopreservation in Horticultural Species 908, 439-446.
Cite This Article
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    Arsène Mahoutondji Doussoh, Justine Sossou Dangou, Arnaud Agbidinoukoun, Serge Sètondji Houedjissin, Corneille Ahanhanzo. (2018). The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin. Journal of Plant Sciences, 6(3), 93-100. https://doi.org/10.11648/j.jps.20180603.13

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    ACS Style

    Arsène Mahoutondji Doussoh; Justine Sossou Dangou; Arnaud Agbidinoukoun; Serge Sètondji Houedjissin; Corneille Ahanhanzo. The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin. J. Plant Sci. 2018, 6(3), 93-100. doi: 10.11648/j.jps.20180603.13

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    AMA Style

    Arsène Mahoutondji Doussoh, Justine Sossou Dangou, Arnaud Agbidinoukoun, Serge Sètondji Houedjissin, Corneille Ahanhanzo. The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin. J Plant Sci. 2018;6(3):93-100. doi: 10.11648/j.jps.20180603.13

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  • @article{10.11648/j.jps.20180603.13,
      author = {Arsène Mahoutondji Doussoh and Justine Sossou Dangou and Arnaud Agbidinoukoun and Serge Sètondji Houedjissin and Corneille Ahanhanzo},
      title = {The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin},
      journal = {Journal of Plant Sciences},
      volume = {6},
      number = {3},
      pages = {93-100},
      doi = {10.11648/j.jps.20180603.13},
      url = {https://doi.org/10.11648/j.jps.20180603.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20180603.13},
      abstract = {Ex situ conservation of genetic resources remains a challenge for preservation of vegetatively propagated species such as sweet potato. The objective of this study was to establish the protocol based on the encapsulation-dehydration technique for short term preservation of endangered sweet potato landraces produced in Benin. Thus, the evaporative dehydration duration on silicagel was previously determinated on the empty beads which were made using alginate sodium (3%) and calcium chloride (1.32M). Then, the young shoots of two sweet potato landraces (Koïdokpon and Dokoui carotte) growing in the screen house were cutted aseptically and desinfected with 10% sodium hypochlorite. The apices were excised on stereoscope and were encapsulated prior their dehydration on silicagel during 5 and 6h. The encapsulated apices were finally conserved in eppendorf tubes at 2°C in batches for 15 days and 90 days. The encapsulated apices were cultured in MS medium supplemented with 0.15 mg/l BAP, 0.2 mg/l NAA, 0.08 mg/l GA3 and 80 mg/l adenine sulfate. The survival and the regeneration rates were then evaluated. At our experimentation condition, the dehydration duration which allowed around 20% water content of the beads was 6h. For the encapsulated apices, the highest survival rates (59.26% and 37.04%) and the highest regeneration rate (37.04% and 11.11%) were recorded respectivelly with the landraces ''Koïdokpon'' and ''Dokoui carotte'' when the apices were dehydrated by 6h and stored for 15 days. The regeneration rates decreased according to the stored duration. Significant difference was noted on the regeneration of apices for the landraces tested. This method can be used to preserve the endangered sweet potato landraces and other species during at least three months without subcultures. It also reduce the cost of conservation in terms of consumables and permit better genotype stability during the storage.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces (Ipomoea batatas Lam) from Benin
    AU  - Arsène Mahoutondji Doussoh
    AU  - Justine Sossou Dangou
    AU  - Arnaud Agbidinoukoun
    AU  - Serge Sètondji Houedjissin
    AU  - Corneille Ahanhanzo
    Y1  - 2018/08/06
    PY  - 2018
    N1  - https://doi.org/10.11648/j.jps.20180603.13
    DO  - 10.11648/j.jps.20180603.13
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 93
    EP  - 100
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20180603.13
    AB  - Ex situ conservation of genetic resources remains a challenge for preservation of vegetatively propagated species such as sweet potato. The objective of this study was to establish the protocol based on the encapsulation-dehydration technique for short term preservation of endangered sweet potato landraces produced in Benin. Thus, the evaporative dehydration duration on silicagel was previously determinated on the empty beads which were made using alginate sodium (3%) and calcium chloride (1.32M). Then, the young shoots of two sweet potato landraces (Koïdokpon and Dokoui carotte) growing in the screen house were cutted aseptically and desinfected with 10% sodium hypochlorite. The apices were excised on stereoscope and were encapsulated prior their dehydration on silicagel during 5 and 6h. The encapsulated apices were finally conserved in eppendorf tubes at 2°C in batches for 15 days and 90 days. The encapsulated apices were cultured in MS medium supplemented with 0.15 mg/l BAP, 0.2 mg/l NAA, 0.08 mg/l GA3 and 80 mg/l adenine sulfate. The survival and the regeneration rates were then evaluated. At our experimentation condition, the dehydration duration which allowed around 20% water content of the beads was 6h. For the encapsulated apices, the highest survival rates (59.26% and 37.04%) and the highest regeneration rate (37.04% and 11.11%) were recorded respectivelly with the landraces ''Koïdokpon'' and ''Dokoui carotte'' when the apices were dehydrated by 6h and stored for 15 days. The regeneration rates decreased according to the stored duration. Significant difference was noted on the regeneration of apices for the landraces tested. This method can be used to preserve the endangered sweet potato landraces and other species during at least three months without subcultures. It also reduce the cost of conservation in terms of consumables and permit better genotype stability during the storage.
    VL  - 6
    IS  - 3
    ER  - 

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Author Information
  • Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, Central Laboratory of Plant Biotechnology and Plant Breeding, Abomey-Calavi, Benin

  • Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Laboratory of Research in Applied Biology, Abomey-Calavi, Benin

  • Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, Central Laboratory of Plant Biotechnology and Plant Breeding, Abomey-Calavi, Benin

  • Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, Central Laboratory of Plant Biotechnology and Plant Breeding, Abomey-Calavi, Benin

  • Department of Genetic and Biotechnology, Faculty of Science and Technology, University of Abomey-Calavi, Central Laboratory of Plant Biotechnology and Plant Breeding, Abomey-Calavi, Benin

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