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Regulation of Phytohormone Biosynthesis Genes by Polycomb-Mediated Histone H3 Lysine 27 Trimethylation in Arabidopsis

Received: 6 August 2018     Accepted: 21 August 2018     Published: 21 September 2018
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Abstract

Histone H3 lysine 27 trimethylation (H3K27me3) catalyzed by polycomb proteins plays a negative role in the regulation of gene expression. Several key developmental genes have been reported to be enriched with H3K27me3 and repressed by polycomb proteins. The objective of this study was to analyze the role of polycomb-mediated H3K27me3 in regulating expression of hormone biosynthesis genes in Arabidopsis. First of all, enrichment of H3K27me3 on hormone biosynthesis genes was investigated systematically. The results showed that genes encoding crucial enzyme tended to be enriched with H3K27me3 while the others were not. And then expression of cytokinin biosynthesis genes in curly leaf (clf) and like heterochromatin protein1 (lhp1) was detected by qPCR. Most of cytokinin biosynthesis genes expression with basal promoter activity was increased in clf and lhp1. Chromatin Immunoprecipitation (ChIP) analysis indicates that H3K27me3 levels on these genes were decreased in clf, suggesting repressive role of CLF and LHP1 in the regulation of these genes. To reveal the role of CLF1 and LHP1 in the dynamics of gene expression, we tested expression of various genes at different time point after IAA treatment. We found that expression of CYP735A2 was increased in clf1 and lhp1 but could be repressed to the levels as in wild type plants, which indicates that CLF1 and LHP1 are not required for IAA-stimulated repression of CYP735A2. The induction of gibberellin 20-oxidase1 (AtGA20Ox1) and gibberellin 20-oxidase2 (AtGA20Ox2) by IAA application was also affected in clf and lhp1. Expression of AtGA20Ox1 and AtGA20Ox2 was firstly induced by IAA treatment and decreased later in wild type plants. However, decrease of AtGA20Ox1 and AtGA20Ox2 expression was earlier in clf and lhp1 which might be caused by overproduction of gibberellins in the mutants. Thus, we propose that the role of the polycomb group proteins is to reduce expression levels of crucial enzymes to minimum, which might stop hormone production.

Published in Journal of Plant Sciences (Volume 6, Issue 4)
DOI 10.11648/j.jps.20180604.12
Page(s) 117-133
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Arabidopsis, Polycomb Protein, H3K27me3, Hormone Biosynthesis

References
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    Yongfeng Hu, Yan Lai, Shufang Fan, Bin Liu. (2018). Regulation of Phytohormone Biosynthesis Genes by Polycomb-Mediated Histone H3 Lysine 27 Trimethylation in Arabidopsis. Journal of Plant Sciences, 6(4), 117-133. https://doi.org/10.11648/j.jps.20180604.12

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    ACS Style

    Yongfeng Hu; Yan Lai; Shufang Fan; Bin Liu. Regulation of Phytohormone Biosynthesis Genes by Polycomb-Mediated Histone H3 Lysine 27 Trimethylation in Arabidopsis. J. Plant Sci. 2018, 6(4), 117-133. doi: 10.11648/j.jps.20180604.12

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    AMA Style

    Yongfeng Hu, Yan Lai, Shufang Fan, Bin Liu. Regulation of Phytohormone Biosynthesis Genes by Polycomb-Mediated Histone H3 Lysine 27 Trimethylation in Arabidopsis. J Plant Sci. 2018;6(4):117-133. doi: 10.11648/j.jps.20180604.12

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  • @article{10.11648/j.jps.20180604.12,
      author = {Yongfeng Hu and Yan Lai and Shufang Fan and Bin Liu},
      title = {Regulation of Phytohormone Biosynthesis Genes by Polycomb-Mediated Histone H3 Lysine 27 Trimethylation in Arabidopsis},
      journal = {Journal of Plant Sciences},
      volume = {6},
      number = {4},
      pages = {117-133},
      doi = {10.11648/j.jps.20180604.12},
      url = {https://doi.org/10.11648/j.jps.20180604.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20180604.12},
      abstract = {Histone H3 lysine 27 trimethylation (H3K27me3) catalyzed by polycomb proteins plays a negative role in the regulation of gene expression. Several key developmental genes have been reported to be enriched with H3K27me3 and repressed by polycomb proteins. The objective of this study was to analyze the role of polycomb-mediated H3K27me3 in regulating expression of hormone biosynthesis genes in Arabidopsis. First of all, enrichment of H3K27me3 on hormone biosynthesis genes was investigated systematically. The results showed that genes encoding crucial enzyme tended to be enriched with H3K27me3 while the others were not. And then expression of cytokinin biosynthesis genes in curly leaf (clf)  and like heterochromatin protein1 (lhp1) was detected by qPCR. Most of cytokinin biosynthesis genes expression with basal promoter activity was increased in clf and lhp1. Chromatin Immunoprecipitation (ChIP) analysis indicates that H3K27me3 levels on these genes were decreased in clf, suggesting repressive role of CLF and LHP1 in the regulation of these genes. To reveal the role of CLF1 and LHP1 in the dynamics of gene expression, we tested expression of various genes at different time point after IAA treatment. We found that expression of CYP735A2 was increased in clf1 and lhp1 but could be repressed to the levels as in wild type plants, which indicates that CLF1 and LHP1 are not required for IAA-stimulated repression of CYP735A2. The induction of gibberellin 20-oxidase1 (AtGA20Ox1) and gibberellin 20-oxidase2 (AtGA20Ox2) by IAA application was also affected in clf and lhp1. Expression of AtGA20Ox1 and AtGA20Ox2 was firstly induced by IAA treatment and decreased later in wild type plants. However, decrease of AtGA20Ox1 and AtGA20Ox2 expression was earlier in clf and lhp1 which might be caused by overproduction of gibberellins in the mutants. Thus, we propose that the role of the polycomb group proteins is to reduce expression levels of crucial enzymes to minimum, which might stop hormone production.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Regulation of Phytohormone Biosynthesis Genes by Polycomb-Mediated Histone H3 Lysine 27 Trimethylation in Arabidopsis
    AU  - Yongfeng Hu
    AU  - Yan Lai
    AU  - Shufang Fan
    AU  - Bin Liu
    Y1  - 2018/09/21
    PY  - 2018
    N1  - https://doi.org/10.11648/j.jps.20180604.12
    DO  - 10.11648/j.jps.20180604.12
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 117
    EP  - 133
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20180604.12
    AB  - Histone H3 lysine 27 trimethylation (H3K27me3) catalyzed by polycomb proteins plays a negative role in the regulation of gene expression. Several key developmental genes have been reported to be enriched with H3K27me3 and repressed by polycomb proteins. The objective of this study was to analyze the role of polycomb-mediated H3K27me3 in regulating expression of hormone biosynthesis genes in Arabidopsis. First of all, enrichment of H3K27me3 on hormone biosynthesis genes was investigated systematically. The results showed that genes encoding crucial enzyme tended to be enriched with H3K27me3 while the others were not. And then expression of cytokinin biosynthesis genes in curly leaf (clf)  and like heterochromatin protein1 (lhp1) was detected by qPCR. Most of cytokinin biosynthesis genes expression with basal promoter activity was increased in clf and lhp1. Chromatin Immunoprecipitation (ChIP) analysis indicates that H3K27me3 levels on these genes were decreased in clf, suggesting repressive role of CLF and LHP1 in the regulation of these genes. To reveal the role of CLF1 and LHP1 in the dynamics of gene expression, we tested expression of various genes at different time point after IAA treatment. We found that expression of CYP735A2 was increased in clf1 and lhp1 but could be repressed to the levels as in wild type plants, which indicates that CLF1 and LHP1 are not required for IAA-stimulated repression of CYP735A2. The induction of gibberellin 20-oxidase1 (AtGA20Ox1) and gibberellin 20-oxidase2 (AtGA20Ox2) by IAA application was also affected in clf and lhp1. Expression of AtGA20Ox1 and AtGA20Ox2 was firstly induced by IAA treatment and decreased later in wild type plants. However, decrease of AtGA20Ox1 and AtGA20Ox2 expression was earlier in clf and lhp1 which might be caused by overproduction of gibberellins in the mutants. Thus, we propose that the role of the polycomb group proteins is to reduce expression levels of crucial enzymes to minimum, which might stop hormone production.
    VL  - 6
    IS  - 4
    ER  - 

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Author Information
  • Department of Bioengineering, Jingchu University of Technology, Jingmen, China

  • Department of Bioengineering, Jingchu University of Technology, Jingmen, China

  • Department of Bioengineering, Jingchu University of Technology, Jingmen, China

  • Department of Bioengineering, Jingchu University of Technology, Jingmen, China

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