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Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat

Received: 16 September 2020     Accepted: 26 May 2021     Published: 21 June 2021
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Abstract

Yam (Dioscorea spp L.) is one of root and tuber crops grown in Ethiopia as source of food and income. It is cultivated mainly in south, southwest, and western part of the country. Two hundred ten yam accessions from ten different geographic origins of major growing areas of the country were used in this study. The sprouted tubers of some accessions were received from research centers where others were directly collected from farmers’ fields during early March, 2010. The collected yam genotypes were planted in complete randomized block design at two research sites (namely at Hawasa and Wonago) which are found under South Agricultural Research Institute in the end of April 2010. All important cultural practices such as staking, weeding and irrigation were done starting from planting till harvesting. Yam leaf samples with of different origin were observed separately using twenty SSR markers. A similarity matrix derived UPGMA cluster analysis based on geographical sites showed nine distinct clustering groups which indicated presence of relationship between genetic distances to that of geographical distance in most of clustering groups. These cluster grouping is supported by Analysis of Molecular Variance (AMOVA) that indicated presence of significance genetic variation of 210 yam accessions within and among ten collection sites.

Published in Journal of Plant Sciences (Volume 9, Issue 3)
DOI 10.11648/j.jps.20210903.14
Page(s) 96-106
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Yam, Genetic Diversity, Geographic Distance, SSR

References
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[5] Borsch, T., Hilu, K. W., Quandt, D., Wilde, V., Neinhuis, C. and Barthlott, W. (2003). Noncoding plastid trnT-trnF sequences reveal a well resolved phylogeny of basal angiosperms. J. Evol. Biol. 16: 558-576.
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[8] Yeh, F., Boyle, T. (1997). Population genetic analy-sis of co-dominant markers and quantitative traits. Belgian Journal of Botany, 129: 157.
[9] Pavlicek, A., Hrda, S. and Flegr, J. (1999). Free tree free ware program for construction of phylogenetic trees on the basis of distance data and bootstrap/Jack Knife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia. Folia. Biologica. 45: 97-99.
[10] Sneath, P, and Sokal, R. (1973). Principles of Numerical Taxonomy Freeman San Francisco.
[11] Pavlicek Nei’s, M. (1978). Estimation of average heterozygosis and genetic distance from a small number of individuals. Genetics, 3 (89). 583–590.
[12] Linda, M., Arshiya, N., and Mario, A. (2009). Assessing Plant Genetic Diversity by Molecular Tools. Department of Aerobiology and Agro chemistry, Tuscia University, Italy.
[13] Morgante, M., and Olivier, A. (1993). PCR-amplified microsatellites as markers in plant genetics. Plant J. 3, 175–182.
[14] Morgante, M., Hanafey, H., and Powell, W. (2002). Microsatellites are preferentially associated with non repetitive DNA in plant genome. Nature Genet 30: 194-200 Tropical Agriculture (Trinidad) 54: 1–13.
[15] Singh, B. D. (2000). Plant Breeding: Principles and Methods. Kalyani Publishers, New Delhi-Ludhiana.
[16] Muluneh, Tamru. (2006). Assessing Diversity in Yams (Dioscorea spp.) from Ethiopia based on, Morphology, AFLP Markers and Tuber Quality, and Farmers management of landraces.
[17] Obidiegwu, J., Kolesnikova-Allen M., Ene-obong, E., Muoneke, C., and Asiedu, A., (2009). SSR markers reveal diversity in Guinea yam (D. cayenensis/D. rotundata) core set. African Journal of Biotechnology Vol. 8 (12), pp. 2730-2739.
[18] Obidiegwu, E., Asiedu, R., Ene-Obong, E., Muoneke, C, and Kolesnikova, M, (2009). Genetic characterization of some water yam (Dioscorea alata L.) accessions in West Africa with simple sequenc repeats. Journal of Food & Agriculture.
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  • APA Style

    Atnafua Bekele, Endashaw Bekele. (2021). Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat. Journal of Plant Sciences, 9(3), 96-106. https://doi.org/10.11648/j.jps.20210903.14

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    ACS Style

    Atnafua Bekele; Endashaw Bekele. Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat. J. Plant Sci. 2021, 9(3), 96-106. doi: 10.11648/j.jps.20210903.14

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    AMA Style

    Atnafua Bekele, Endashaw Bekele. Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat. J Plant Sci. 2021;9(3):96-106. doi: 10.11648/j.jps.20210903.14

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  • @article{10.11648/j.jps.20210903.14,
      author = {Atnafua Bekele and Endashaw Bekele},
      title = {Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat},
      journal = {Journal of Plant Sciences},
      volume = {9},
      number = {3},
      pages = {96-106},
      doi = {10.11648/j.jps.20210903.14},
      url = {https://doi.org/10.11648/j.jps.20210903.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20210903.14},
      abstract = {Yam (Dioscorea spp L.) is one of root and tuber crops grown in Ethiopia as source of food and income. It is cultivated mainly in south, southwest, and western part of the country. Two hundred ten yam accessions from ten different geographic origins of major growing areas of the country were used in this study. The sprouted tubers of some accessions were received from research centers where others were directly collected from farmers’ fields during early March, 2010. The collected yam genotypes were planted in complete randomized block design at two research sites (namely at Hawasa and Wonago) which are found under South Agricultural Research Institute in the end of April 2010. All important cultural practices such as staking, weeding and irrigation were done starting from planting till harvesting. Yam leaf samples with of different origin were observed separately using twenty SSR markers. A similarity matrix derived UPGMA cluster analysis based on geographical sites showed nine distinct clustering groups which indicated presence of relationship between genetic distances to that of geographical distance in most of clustering groups. These cluster grouping is supported by Analysis of Molecular Variance (AMOVA) that indicated presence of significance genetic variation of 210 yam accessions within and among ten collection sites.},
     year = {2021}
    }
    

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    T1  - Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat
    AU  - Atnafua Bekele
    AU  - Endashaw Bekele
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    N1  - https://doi.org/10.11648/j.jps.20210903.14
    DO  - 10.11648/j.jps.20210903.14
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
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    EP  - 106
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20210903.14
    AB  - Yam (Dioscorea spp L.) is one of root and tuber crops grown in Ethiopia as source of food and income. It is cultivated mainly in south, southwest, and western part of the country. Two hundred ten yam accessions from ten different geographic origins of major growing areas of the country were used in this study. The sprouted tubers of some accessions were received from research centers where others were directly collected from farmers’ fields during early March, 2010. The collected yam genotypes were planted in complete randomized block design at two research sites (namely at Hawasa and Wonago) which are found under South Agricultural Research Institute in the end of April 2010. All important cultural practices such as staking, weeding and irrigation were done starting from planting till harvesting. Yam leaf samples with of different origin were observed separately using twenty SSR markers. A similarity matrix derived UPGMA cluster analysis based on geographical sites showed nine distinct clustering groups which indicated presence of relationship between genetic distances to that of geographical distance in most of clustering groups. These cluster grouping is supported by Analysis of Molecular Variance (AMOVA) that indicated presence of significance genetic variation of 210 yam accessions within and among ten collection sites.
    VL  - 9
    IS  - 3
    ER  - 

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Author Information
  • South Agricultural Research Institute, Hawassa Agricultural Research Centre, Hawassa, Ethiopia

  • Addis Ababa Universities, Microbial, Cellular and Molecular Biology Department, Addis Ababa, Ethiopia

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