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Polygonatum Kingianum Genome Size Estimation by Flow Cytometry

Received: 7 October 2022     Accepted: 1 November 2022     Published: 4 November 2022
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Abstract

Polygonatum kingianum, an herb of Polygonatum in Liliaceae, is a kind of Chinese herbal medicine with homology of medicine and food. Polygonatum kingianum has extensive scientific research, but its genome size is seldom studied. In order to enrich the genome data of P. kingianum, this study was based on flow cytometry to detect P. kingianum collected from different areas and estimate its genome size. We use corn as the internal reference plant and rice as the internal reference plant to correct the data, and control the CV value below 5.0% to ensure the accuracy of the results. The results showed that the genome size of P. kingianum was about 6.90±0.41 pg. Moreover, high-quality results have CV values of approximately 1.0%–2.0%, and the conventional results have CV values of approximately 3.0%. The average coefficient of variation in this study is 2.5%-3.5%; therefore, the genome size of the three populations in this study has high reliability. In a word, this study provides the first estimate of the genome size of P. kingianum. In the future, these results can be used to establish sequence data and provide a basis for determining the whole genome of P. kingianum. Moreover, these results provide a basis for selecting research materials for future work on P. kingianum genetic evolution and molecular breeding.

Published in Journal of Plant Sciences (Volume 10, Issue 6)
DOI 10.11648/j.jps.20221006.11
Page(s) 203-208
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Flow Cytometry (FCM), Genome Size, Polygonatum Kingianum

References
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Cite This Article
  • APA Style

    Xiao-Lei Chen, Kun Cong, Zhe Xu, Hua-Li Qian, Hao Chen, et al. (2022). Polygonatum Kingianum Genome Size Estimation by Flow Cytometry. Journal of Plant Sciences, 10(6), 203-208. https://doi.org/10.11648/j.jps.20221006.11

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    ACS Style

    Xiao-Lei Chen; Kun Cong; Zhe Xu; Hua-Li Qian; Hao Chen, et al. Polygonatum Kingianum Genome Size Estimation by Flow Cytometry. J. Plant Sci. 2022, 10(6), 203-208. doi: 10.11648/j.jps.20221006.11

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    AMA Style

    Xiao-Lei Chen, Kun Cong, Zhe Xu, Hua-Li Qian, Hao Chen, et al. Polygonatum Kingianum Genome Size Estimation by Flow Cytometry. J Plant Sci. 2022;10(6):203-208. doi: 10.11648/j.jps.20221006.11

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  • @article{10.11648/j.jps.20221006.11,
      author = {Xiao-Lei Chen and Kun Cong and Zhe Xu and Hua-Li Qian and Hao Chen and Jian-Yun Su and Lei Zhang and Jia-Hong Dong and Peng-Zhang Ji},
      title = {Polygonatum Kingianum Genome Size Estimation by Flow Cytometry},
      journal = {Journal of Plant Sciences},
      volume = {10},
      number = {6},
      pages = {203-208},
      doi = {10.11648/j.jps.20221006.11},
      url = {https://doi.org/10.11648/j.jps.20221006.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20221006.11},
      abstract = {Polygonatum kingianum, an herb of Polygonatum in Liliaceae, is a kind of Chinese herbal medicine with homology of medicine and food. Polygonatum kingianum has extensive scientific research, but its genome size is seldom studied. In order to enrich the genome data of P. kingianum, this study was based on flow cytometry to detect P. kingianum collected from different areas and estimate its genome size. We use corn as the internal reference plant and rice as the internal reference plant to correct the data, and control the CV value below 5.0% to ensure the accuracy of the results. The results showed that the genome size of P. kingianum was about 6.90±0.41 pg. Moreover, high-quality results have CV values of approximately 1.0%–2.0%, and the conventional results have CV values of approximately 3.0%. The average coefficient of variation in this study is 2.5%-3.5%; therefore, the genome size of the three populations in this study has high reliability. In a word, this study provides the first estimate of the genome size of P. kingianum. In the future, these results can be used to establish sequence data and provide a basis for determining the whole genome of P. kingianum. Moreover, these results provide a basis for selecting research materials for future work on P. kingianum genetic evolution and molecular breeding.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Polygonatum Kingianum Genome Size Estimation by Flow Cytometry
    AU  - Xiao-Lei Chen
    AU  - Kun Cong
    AU  - Zhe Xu
    AU  - Hua-Li Qian
    AU  - Hao Chen
    AU  - Jian-Yun Su
    AU  - Lei Zhang
    AU  - Jia-Hong Dong
    AU  - Peng-Zhang Ji
    Y1  - 2022/11/04
    PY  - 2022
    N1  - https://doi.org/10.11648/j.jps.20221006.11
    DO  - 10.11648/j.jps.20221006.11
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 203
    EP  - 208
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.20221006.11
    AB  - Polygonatum kingianum, an herb of Polygonatum in Liliaceae, is a kind of Chinese herbal medicine with homology of medicine and food. Polygonatum kingianum has extensive scientific research, but its genome size is seldom studied. In order to enrich the genome data of P. kingianum, this study was based on flow cytometry to detect P. kingianum collected from different areas and estimate its genome size. We use corn as the internal reference plant and rice as the internal reference plant to correct the data, and control the CV value below 5.0% to ensure the accuracy of the results. The results showed that the genome size of P. kingianum was about 6.90±0.41 pg. Moreover, high-quality results have CV values of approximately 1.0%–2.0%, and the conventional results have CV values of approximately 3.0%. The average coefficient of variation in this study is 2.5%-3.5%; therefore, the genome size of the three populations in this study has high reliability. In a word, this study provides the first estimate of the genome size of P. kingianum. In the future, these results can be used to establish sequence data and provide a basis for determining the whole genome of P. kingianum. Moreover, these results provide a basis for selecting research materials for future work on P. kingianum genetic evolution and molecular breeding.
    VL  - 10
    IS  - 6
    ER  - 

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Author Information
  • School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine, Kunming, China

  • Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, China

  • School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine, Kunming, China

  • School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine, Kunming, China

  • School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine, Kunming, China

  • School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine, Kunming, China

  • School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine, Kunming, China

  • School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine, Kunming, China

  • School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine, Kunming, China

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